Using a new laser imaging technology
laser imaging technology .scientists can view the status of small animals in real time. The technology is based on light and ultrasound, the resolution is enough to enable scientists to see the animal organs, mobile blood, the proliferation of melanoma cells, and efficient operation of neural networks. In this way, the researchers can monitor the spread of drugs in animals in the process of understanding the response of different organs of drugs.
The image shows the mouse organ image generated by photoacoustic imaging. The technology combines light with ultrasound to generate a complete tomogram in real time.laser imaging technology
The image shows the mouse intestinal image generated by photoacoustic imaging.
The study was conducted by Duke University and the California Institute of Technology. The technology is called “single pulse photoacoustic computed tomography” (referred to as SIP-PACT), the use of light microscopy and ultrasound imaging technology to observe the animal’s in vivo condition. Researchers say there is always a limitation of image resolution and scanning speed for live scans of small animals, and this new technology can solve this problem.
It can be generated in real time in vivo animal tomography, adult mice, for example, can generate 50 per second complete tomography results. “Photo-acoustic imaging technology can be used to generate a complete tomographic image of a small animal’s body in real time,” said Junjie Yao, assistant professor of biomedical engineering at Duke University, co-author of the study. “Using this technology, People can easily monitor the distribution of drugs in animals, as well as the response of different organs to drugs.laser imaging technology
Photoacoustic imaging technology integrates multiple imaging technologies on the same platform. Traditional light microscopy can quickly generate high-resolution images that absorb, reflect, or diverge light wavelengths through different tissues (ie, color), reflecting the body’s internal details. For example, melanin can absorb near infrared light, and the blood reaction to light depends on the level of blood oxygen. However, since most of the light is scattered when it penetrates the tissue, the imaging depth is only a few millimeters. In contrast, ultrasound can easily penetrate the body tissue, so we can observe more in-depth. But the ultrasound can not determine the chemical composition of the organization, it can not be the same as the light, to provide us with important diagnosis and treatment information.
Nuclear magnetic resonance imaging (MRI) can also observe the internal situation of the tissue, but with the help of a strong magnetic field, and the length of the image generated from a few seconds to several minutes, X-ray and positron emission tomography (PET ) Will produce a lot of radiation, can not be used for long time observation, but the use of photoacoustic imaging technology is a powerful and short laser pulse, can ensure the safety of the premise, so that cells emit ultrasound, and then penetrate the body tissue.
In this latest study, Dr. Yao and Dr. Lihong Wang of the California Institute of Technology have also increased the speed and scanning range of photoacoustic imaging technology. They set up a ring ultrasonic detector and a set of fast data acquisition system, the use of triangulation to determine the small animals in the body of the source of ultrasound, and ultimately, improved imaging technology can be deep inside the biological tissue five centimeters, resolution Daya Mm, while retaining the information provided by traditional light microscopy.
“This is equivalent to the summer afternoon afternoon to collect the sun into a nail into a large area, and then within a nanosecond launch.” Dr. Yao explained. He has been working on this technology for nearly a decade. “After the laser hit the cells, the energy it carries makes the cells warm up slightly and the volume swells immediately, resulting in an ultrasonic wave, which is like moving the object slowly and striking it, making it the difference between vibrations, We can understand the full range of information in the body, each laser pulse will not miss any details, you can real-time monitoring of biological dynamics, such as heart beating, arterial expansion, different organizational functions.
Researchers used the technique to track the process of melanoma cells in the mouse intravascular transfer, while shooting the entire brain network high-speed operation of the video. “This technology is very powerful because it does not need to inject any contrast agent,” Dr. Yao said, “so that we can determine that changes in the body are not caused by external causes. We believe that this technology in the pre-clinical imaging and clinical diagnosis The field has great potential. “laser imaging technology